In the present study, we developed a quantitative real-time RT-PCR assay to measure TGF-β mRNA transcription and used the well-known synthetic glucocorticoid, triamcinolone, as a model compound to generate baseline immune function data.
Fast reagents can be a more cost-effective solution than standard cycling reagents for your real-time PCR experiments. Today’s Fast cycling reagents have been optimized to deliver performance equal to or better than traditional PCR approaches, with smaller sample and reagent volumes.
Both TGF-β and β-actin primers were further optimized for real-time PCR by amplifying different concentrations (300–500 nM) of each primer set with known amounts of each target. PCR and gel electrophoresis analysis were used to verify specificity of primer pairs for each target gene sequence.
At Life Technologies, we pioneered standard and Fast real-time PCR and have applied over 20 years of experience to bring you the best-performing products based on the latest advances in real-time PCR technology. Our newest additions to our real-time PCR portfolio will enable you to obtain superior data, in less than half the time.
The FastPCR software is an integrated tools environment that provides comprehensive and professional facilities for designing any kind of PCR primers for standard, long distance, inverse, real-time PCR (TaqMan, LUX-primer, Molecular Beacon, Scorpion), multiplex PCR, Xtreme Chain Reaction (XCR®), group-specific (universal primers for genetically related DNA sequences) or unique (specific ...
www.appliedbiosystems.com Figure 7.Comparison of TaqMan ®Gene Expression Assays and TaqManPre-Developed Assay Reagents (8 technical replicates) on the 7500 Fast System.Left: Assay set run using fast mode (run time: 37 minutes). Right: The same assay set run using standard mode (run time: 1 hour, 20 minutes). Note that the CT for the GAPDH assay set (blue) is earlier in fast
Are you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification
A good in silico PCR program must be able to handle degenerate primers or probes including those with 5′ or 3′ tail sequences and single nucleotide polymorphisms (SNPs). In addition, the program should be compatible with bisulfite-treated DNA containing only methylated cytosine and highly degraded and chemically modified DNA from ancient herbarium and mummies.
Polymerase chain reaction (PCR) is one of the most important laboratory techniques used in molecular biology, genetics and molecular diagnostics. The success of a PCR-based method largely depends on the correct nucleic acid sequence analysis in silico prior to a wet-bench experiment. Here, we report …
Under current standard cycling conditions, a typical run takes approximately 2 hours, limiting the number of researchers or runs on that instrument to only four per 8-hour day. Running your current real-time PCR instrument in Fast mode will triple the number of researchers or runs on that same instrument by reducing your run times ...
Fast reagents can be a more cost-effective solution than standard cycling reagents for your real-time PCR experiments. Today’s Fast cycling reagents have been optimized to deliver performance equal to or better than traditional PCR approaches, with smaller sample and reagent volumes.
Under current standard cycling conditions, a typical run takes approximately 2 hours, limiting the number of researchers or runs on that instrument to only four per 8-hour day. Running your current real-time PCR instrument in Fast mode will triple the number of researchers or runs on that same instrument by reducing your run times ...
Fast reagents can be a more cost-effective solution than standard cycling reagents for your real-time PCR experiments. Today’s Fast cycling reagents have been optimized to deliver performance equal to or better than traditional PCR approaches, with smaller sample and reagent volumes.