Never ever use the coarse adjustment knob with the high power objective. If you turn the coarse adjustment under high power, you will most likely break the slide, and possibly the objective. Using the low power or scanning objective, look through the ocular and see if you can visualize the specimen.
Mar 07, 2021 · Why should you never use the coarse adjustment knob to focus under high power? NEVER USE THE COARSE FOCUS KNOB ON HIGH POWER! The high power lens should be very close to your slide when in proper focus. If you turn the coarse adjustment knob while on high power, the objective could easily break your slide.
Oct 06, 2018 · The coarse adjustment knob should never be used when viewing in high power with a compound microscope. When in high power use the coarse adjustment (the knob smaller than the … coarse adj.) to more accurately focus on the subject. Coarse and fine adjustment The coarse adjustment knob should only be used with the lowest power objective lens.
Feb 08, 2022 · NEVER USE THE COARSE ADJUSTMENT KNOB when ON HIGH POWER. This can cause damage to the slide, the objective lens or both. When using the high power objective you should not use the? Use the coarse focus to raise the stage all the way up until it blocks. The objective at the highest position, the 4x objective should of course not touch the slide.
Mar 29, 2020 · Why should I not use the coarse focus knob for high-power objectives? First, the image moves in and out of focus too quickly, so that it is difficult to precisely adjust the focus. Second, you run the risk of crashing the objective into the slide. Use the coarse focus only with the 4x low power objective. Complete answer to this is here.
Important Note: Before switching to high power, you should always position the specimen in the center of the field of view and use the fine adjust to sharpen the focus of the image. Never use the coarse adjustment when using high power. Doing so could break the slide or the microscope!
Coarse and fine adjustment The coarse adjustment knob should only be used with the lowest power objective lens. … Using the coarse focus with higher lenses may result in crashing the lens into the slide.
Immersion oil is a transparent liquid with a high refractive index, which allows the light to pass directly into the objective lens, enhancing the resolution. DO NOT USE THE COARSE ADJUSTMENT WITH THE OIL IMMERSION OBJECTIVE. ONLY USE THE FINE OBJECTIVE, BECAUSE YOU CAN BREAK THE LENS.
What happens if you try to use the coarse adjustment when the 10X lens is in place? The focus of the specimen would be unclear with a high objective power and the stage lifted high due to the coarse adjustment.
NEVER USE THE COARSE ADJUSTMENT KNOB when ON HIGH POWER. This can cause damage to the slide, the objective lens or both.
Use the coarse focus to raise the stage all the way up until it blocks. The objective at the highest position, the 4x objective should of course not touch the slide.
The fine-adjustment knob on a light microscope focuses by moving the lens slowly and is used to focus on the specimen more clearly. It is used with the high-power objective lenses. The course-adjustment knob focuses by moving the lens quickly and is used to find the specimen your are observing.
If you focus the high power objectives with the coarse focus knob, then you risk slamming the objective into the slide, risking the damage of both. Some high power objectives are spring-loaded, with a front-part that retracts when touched by the slide. This is a protective mechanism.
The 4x objective also allows you to center the slide better, because it gives you a better overview. The 4x also has the greatest depth of field and this means that the whole specimen (top and bottom parts) are in focus. If you start with a high power objective and then start focusing, then you don’t know if you are focusing at the actual specimen or on the dust which is on the top of the cover glass.
If you look at a specimen under high power and then directly exchange the slide, you run the risk of losing focus again . The thickness of the mounting medium is not always the same and also the position of the specimen in the mounting medium can be different. Using the coarse (and even the fine focus) will take longer than starting again with the 4x objective and the coarse focus.