Replication occurs in three major steps: the opening of the double helix and separation of the DNA strands, the priming of the template strand, and the assembly of the new DNA segment.
The synthesis of a primer is necessary because the enzymes that synthesize DNA, which are called DNA polymerases, can only attach new DNA nucleotides to an existing strand of nucleotides. The primer therefore serves to prime and lay a foundation for DNA synthesis.
DNA replication is said to be semiconservative because each newly made DNA molecule has one original and one new strand of DNA. A laboratory technique used to replicate, and thus amplify, a specific DNA segment.
DNA polymeraseCytosine—one of the nucleotides that make up DNA and RNA. DNA polymerase—enzyme which assembles nucleotides to build a strand of DNA, using a complementary strand as a template.
So, the correct option is 'RNA polymerase'
This type of replication is called bi-directional. Most organisms, including mammals, use bi-directional replication. There are four basic components required to initiate and propagate DNA synthesis. They are: substrates, template, primer and enzymes.
Replication is called semiconservative because at the time of replication, in each of the two copies of the DNA, one of the strands of DNA is old and conserved and one is newly formed.
DNA replication is a semi-conservative process, because when a new double-stranded DNA molecule is formed: One strand will be from the original template molecule. One strand will be newly synthesised.
DNA replication: A complex process whereby the 'parent' strands of DNA in the double helix are separated, and each one is copied to produce a new (daughter) strand. This process is said to be 'semiconservative' because one strand from each parent is conserved and remains intact after replication has taken place.
Enzymes involved in DNA replication are:Helicase (unwinds the DNA double helix)Gyrase (relieves the buildup of torque during unwinding)Primase (lays down RNA primers)DNA polymerase III (main DNA synthesis enzyme)DNA polymerase I (replaces RNA primers with DNA)Ligase (fills in the gaps)
Step 1: Replication Fork Formation. Before DNA can be replicated, the double stranded molecule must be “unzipped” into two single strands. ... Step 2: Primer Binding. The leading strand is the simplest to replicate. ... Step 3: Elongation. ... Step 4: Termination.
Which of the following protein does not involve in the initiation of replication? Explanation: In eukaryotic replication, more than 20 proteins take part out of that the initiation of replication takes place in the presence of helicase, primase, SSB, DnaC, DnaA.