Antibiotic susceptibility testing is a very important step to monitor antimicrobial resistance. It guides the clinician to select the best antimicrobial agent. To acquire information on microorganisms of public health importance.
It can be useful in monitoring antimicrobials and for the selection of proper antibacterial agents. It doesn’t require special equipment to perform and can be interpreted by all medical personnel.
The organism to be tested must be incubated overnight in broth and must be compared with the 0.5 McFarland Turbidity standard. Mueller- Hinton agar must be used as it does not inhibit sulphonamides and ensures reproducibility and with composition and PH of the medium. The agar when poured on Petri dishes should be 4mm.
It is not considered a gold standard test as it is only for screening the susceptibility pattern of the organisms according to the CLSI guideline.