why should the course focus never be used with the 40x objective?

by Clementina Walker 5 min read

Use ONLY the fine focus control when focusing the higher power objectives (20X, 40X, 100X) on a slide. The course focus control is too course for focusing with these objectives. Objectives are fragile and must not be rammed into slides.

If you focus the high power objectives with the coarse focus knob, then you risk slamming the objective into the slide, risking the damage of both. Some high power objectives are spring-loaded, with a front-part that retracts when touched by the slide. This is a protective mechanism.

Full Answer

How do I use the “coarse focus” and “40x” objectives?

Reason 1: protect the objectives from damage. If you focus the high power objectives with the coarse focus knob, then you risk slamming the objective into the slide, risking the damage of both. Some high power objectives are spring-loaded, with a …

How do you use a 10x and 40x objective?

Mar 29, 2020 · Why should I not use the coarse focus knob for high-power objectives? First, the image moves in and out of focus too quickly, so that it is difficult to precisely adjust the focus. Second, you run the risk of crashing the objective into the slide.

What is the difference between course focus and fine focus control?

When working with the 40x or 100x objective lens which focus knobs can you use safely? 4. Use ONLY the fine focus control when focusing the higher power objectives (20X, 40X, 100X) on a slide. The course focus control is too course for focusing with these objectives. Objectives are fragile and must not be rammed into slides.

Should the slide be in focus after focusing at 4x?

Mar 07, 2021 · The objective at the highest position, the 4x objective should of course not touch the slide. When would you use a fine adjustment knob? Fine Adjustment Knob – This knob is inside the coarse adjustment knob and is used to bring the specimen into sharp focus under low power and is used for all focusing when using high power lenses.

Why should you never use the coarse adjustment knob with the 40X objective?

NEVER USE THE COARSE FOCUS KNOB ON HIGH POWER! The high power lens should be very close to your slide when in proper focus. If you turn the coarse adjustment knob while on high power, the objective could easily break your slide.

Can you use coarse focus with the 40X objective?

Bring the 40x objective into position and focus only using the “fine focus” knob. Remember: The 4x objective is the only time you will use the “coarse focus” knob.

Why should you never use the coarse focus knob when performing an oil immersion?

Never use the coarse adjustment knob when focusing with the high-power or oil immersion objectives in place as this can damage the objective or the slide.Dec 14, 2021

Which focusing knob is never used with the 40X objective?

DO NOT USE THE COARSE ADJUSTMENT KNOB WHEN FOCUSING UNDER THE 40X OBJECTIVE! ONLY USE THE FINE ADJUSTMENT KNOB!

What is the 40x objective lens used for?

High Power Objective Lens (40x) The high-powered objective lens (also called “high dry” lens) is ideal for observing fine details within a specimen sample.Mar 16, 2020

Can you use immersion oil with 40x objective?

The air space between the slide and lens can be replaced by a drop of immersion oil which has a different refractive index than air. c. The use of oil immersion is an absolute necessity when using the 40X objective.

What are the dangers of using the coarse adjustment knob when focusing under high power?

Coarse and fine adjustment The coarse adjustment knob should only be used with the lowest power objective lens. Once it is in focus, you will only need to use the fine focus. Using the coarse focus with higher lenses may result in crashing the lens into the slide.

Why is it important to look on from the side while you turn the coarse focus knob to raise the stage?

Answer: It is important because moving the stage with the coarse adjustment knob can break the lens.Feb 10, 2020

When should you use the fine focus knob?

Use the Fine Focus Knob to bring the image into focus. When using 10x and 40x objectives, use ONLY the fine focus knob so that you don't scratch a lens or break a slide. Always carry your microscope with one hand under the base and one hand on the arm.

What should you do if you Cannot focus the specimen at 40X?

If you cannot focus on your specimen, repeat steps 3 through 5 with the higher power objective lens in place. Do not let the objective lens touch the slide! When finished, lower the stage, click the low power lens into position and remove the slide.

Why should the fine focus knob typically be used with the 10x and 40X objective lenses?

Why should the fine focus knob typically be used with the 10x and 40x objective lenses? The slide should be close to in focus after focusing at the 4X objective. Focusing with the course focus knob would result in too large a change in focus.

How do you use a 40X microscope?

0:092:45How to Focus a Microscope & How the Field of View Changes - YouTubeYouTubeStart of suggested clipEnd of suggested clipBegin by turning your microscope on and adjusting the light intensity to about five or six thenMoreBegin by turning your microscope on and adjusting the light intensity to about five or six then place your slide in the clamp on the stage. And center the specimen over the condenser.

Quesiton : Why should you never use the coarse adjustment knob on high power?

Why should you never use the coarse adjustment knob on high power? The coarse adjustment knob should never be used when viewing in high power with a compound microscope. When in high power use the coarse adjustment (the knob smaller than the coarse adj.) to more accurately focus on the subject.

Answer: Your hands

Why should you never use the coarse adjustment knob on high power? The coarse adjustment knob should never be used when viewing in high power with a compound microscope. When in high power use the coarse adjustment (the knob smaller than the coarse adj.) to more accurately focus on the subject.