Nov 16, 2016 · Question 2 1 / 1 pts Which of the following best describes Streptococcus bovis? Gram-positive, helix-shaped Gram-negative, coccus-shaped Gram-negative, helix-shaped Correct! ... Course Hero is not sponsored or endorsed by any college or university. ...
Jun 12, 2015 · Question 13 1 / 1 pts Which of the following best describes Streptococcus bovis ? Question 14 1 / 1 pts What domain does Streptococcus pharyngitis belong to ? Question 15 1 / 1 pts True or False : ... Course Hero is not sponsored or endorsed by any college or university. ...
As this case illustrates, in the unusual setting of a Streptococcus bovis infection, it is necessary to timely and carefully rule out occult colon cancer and other malignancies during hospitalization and, if a tumor is not found, to schedule endoscopic follow-up. ... Case presentation: We describe the case of a 51-year-old man who was admitted ...
Nov 18, 2017 · 3. Staphlo-Grapelike clusters; Staphylococcus epidermidis, Staphyloccocus aureus 4.Describe the four (4) different stages of the bacterial population growth curve: Lag phase: bacteria adapt themselves to growth conditions. This is the period where the individual bacteria are maturing and not yet able to divide and where the synthesis of RNA, enzymes and other …
Principle#N#The purpose of determining the group antigen of ß-hemolytic streptococci is identify the species or species/group of streptococci as originally described by Rebecca Lancefield. Acid extraction is used to remove the serogroup from the cell.
Principle#N#The purpose of growing the unknown strains on agar containing 5% sucrose is to determine the capacity of the strains to form extracellular polysaccharide (levans or dextrans) in the broth.
The selective agent bile, inhibits most gram positive bacteria . The enterococci and Streptococcus bovis will grow. Esculin in the medium is hydrolyzed to esculetin and dextrose. The esculetin reacts with ferric chloride in the media to form a black-brown color.
If the bacteria acidify the carbohydrate, the pH will change and the indicator (brom cresol purple) will turn yellow.
Principle#N#The purpose of the bile solubility test is to aid in the differentiation of S. pneumonia e from all other alpha-hemolytic streptococci. Sodium deoxycholate (2%) acts on the cell wall of pneumococci resulting in lysis.
The purpose of the CAMP test is to aid in the identification of nonhemolytic group B streptococci and other ß-hemolytic streptococci.
The hemolytic reaction is determined on agar media containing 5% animal blood. The most commonly used base medium is trypticase soy agar and the most commonly used blood is sheep blood. The reason for the use of trypticase soy base is that it supports the growth of all the bacteria listed in Table 2. Other base media may be substituted if control strains of all genera are tested for growth. Sheep blood is used because of the convenience in testing throat swabs for ß-hemolytic streptococci. Sheep blood does not support the growth of Haemophilus haemolyticus which appears similar to streptococci on agar containing rabbit, horse, or human blood.
The genus Streptococcus is a complex group causing a wide range of diseases such as: rheumatic fever, impetigo, pharyngitis, laryngitis, toxic shock syndrome, scarlet fever, and endocarditis. Streptococci are often classified based on hemolysis which can be seen by their reaction on blood agar.
Staphylococcus species are normal flora widespread over the body surface. They are also important pathogens. Some of the most common diseases caused by Staphylococcus species include: impetigo, toxic shock syndrome, bacteremia, endocarditis, folliculitis furuncle (boils), and osteomyelitis (bone abscesses). Many species of Staphylococcus have the ability to form biofilms which can then colonize structures such as medical catheters, stents, heart valves, prostheses, shunts, and valves.
Inoculate a loop-full of organism into the tube. Chose a well isolated colony. Ideally you should incubate the tube at 35°C for 4 hours checking every 30 minutes for clot formation.
A negative result is indicated by no growth after 72 hours. Enterococcus spp. typically changes the media color within 24 hours.
Staphs are Gram positive. McFarland Standard. Dilute your organism in a tube of sterile water to obtain a turbidity equivalent to a 0.5 McFarland test standard. Hold your diluted tube and the 0.5 McFarland test standard against the black-lined McFarland reference card to accurately rate the turbidity. Mac.
Four main families with numerous genera and species comprise the Gram negative enteric: Enterobacteriacea, Pseuodmonadaceae, Vibrionaceae, and Camplyobacteraceae. You will only be working with organisms from the first two families.
Colonies capable of utilizing citrate as a carbon source produce a local increase in pH, changing the color of the medium from green to blue. Only citrate positive organisms will grow on this medium.