what part of the time course has the highest rate of enzyme activity

How do you find the highest rate of enzyme activity?

Consider that the rate of enzyme activity is related to the slope of the line, Δy/Δx (the "rise" over the "run"), in μmol/mL • min, with the steepest slope indicating the highest rate of enzyme activity. What part of the time course had the highest rate of enzyme activity? between 5 and 20 minutes

Why does enzyme activity decrease at the end of time course?

As the enzyme activity slows down (when the slope reaches zero), there is no additional being released because the cells have used up the substrate. The rate of product transport reaches its maximum when the slope reaches zero, and that is why the most is released at the end of the time course.

What is the classical approach to enzyme kinetics?

Time course enzyme kinetics The classical approach to enzyme kinetics is focused on initial reaction rates. In assays enzymes are mixed with substrate at known concentrations and the rate of the catalyzed reaction is followed.

How do you determine the optimum pH for enzyme activity?

The optimum pH is usually determined experimentally because of the difficulty in characterizing the active sites of enzyme. The profiles of enzyme reaction rate for each pH often become bell shaped. The typical pH dependence of enzyme reaction rate is shown in Figure 6.

What is your best option for increasing the rate of the reaction?

What is your best option for increasing the rate of the reaction? Increase the enzyme concentration. If an enzyme is saturated with substrate, and it is operating at optimum pH and optimum temperature, there is very little that can be done except to increase the enzyme concentration.

Which action would most likely increase the rate of an enzyme controlled reaction during an experiment?

Answers. If the concentration of the substrate is low, increasing its concentration will increase the rate of the reaction. An increase in the amount of enzyme will increase the rate of the reaction (provided sufficient substrate is present).

Which of the following can cause enzyme activity to either increase or decrease?

chapter 23QuestionAnswerWhich of the following can cause enzyme activity to either increase or decrease?both a and b,(ph and temp?.)What term is used to describe a species which affects the functioning of an allosteric enzyme?regulator31 more rows

Which of the following is the most logical way to regulate an enzymatic pathway if you are trying to keep a constant level of product around?

Which of the following is the most logical way to regulate an enzymatic pathway if you are trying to keep a constant level of product around? The final product inhibits the first enzyme.

How does time affect enzyme activity?

The longer an enzyme is incubated with its substrate, the greater the amount of product that will be formed. However, the rate of formation of product is not a simple linear function of the time of incubation. All proteins suffer denaturation, and hence loss of catalytic activity, with time.

How the active site of an enzyme causes a high rate of reaction?

When an enzyme binds its substrate, it forms an enzyme-substrate complex. This complex lowers the activation energy of the reaction and promotes its rapid progression by providing certain ions or chemical groups that actually form covalent bonds with molecules as a necessary step of the reaction process.

What increases enzyme activity?

Temperature: Raising temperature generally speeds up a reaction, and lowering temperature slows down a reaction. However, extreme high temperatures can cause an enzyme to lose its shape (denature) and stop working. pH: Each enzyme has an optimum pH range. Changing the pH outside of this range will slow enzyme activity.

Which of the following will increase enzyme activity quizlet?

( ) Enzyme activity increases as substrate concentration increases until the maximum rate is achieved.

Which of the following is true of an enzyme that is operating at its maximum rate?

Which of the following is true of an enzyme that is operating at its maximum rate? The substrate-binding sites on the enzyme molecules are fully occupied.

What is the most common process by which enzyme activity is regulated?

The most common mode of enzyme regulation is by protein phosphorylation-dephosphorylation catalyzed by protein kinases and phosphoprotein phosphatases, respectively Krauss (2001a), Krauss (2001b). It is through phosphorylation that protein and enzyme function is regulated in response to extracellular stimuli.

Which of the following are mechanisms by which to regulate the activity of an enzyme?

Genetic control, compartmentalization, regulation of substrate concentration, degradation, alteration of environmental factors like pH, and processing of zymogens are all mechanisms that can be used to regulate enzyme activity (induction and repression).

What regulates enzyme activity in metabolic pathways?

Feedback Inhibition in Metabolic Pathways Cells have evolved to use feedback inhibition to regulate enzyme activity in metabolism, by using the products of the enzymatic reactions to inhibit further enzyme activity.

What are the active sites of enzymes?

The active sites of enzyme consist of amino acids with some ionic groups, such as carboxyl group, amino group, thiol group, imidazole group, phenolic hydroxyl group, and so on.

What is enzyme activity?

Enzyme activity is measured in units which indicate the rate of reaction catalysed by that enzyme expressed as micromoles of substrate transformed (or product formed) per minute. An enzyme unit is the amount of enzyme that will catalyse the transformation of 1 μmol of substrate/min under specified conditions of pH and temperature. The specific activity of an enzyme is expressed as the number of units per milligram of protein.

What enzymes are used to determine the location of renal tubular injury?

Urinary enzyme activity has also been used to determine the presence and location of renal tubular injury. A list of the most commonly used enzymes is presented in Table 7.

How is enzyme activity demonstrated?

In general, enzyme activity is demonstrated by fluorescence microscopy as follows. A substrate is offered to the enzyme, which is allowed to act on the substrate to obtain a reaction product which is localized at the site of enzyme activity and is either fluorescent or easily rendered so.

How to find specific activity of enzyme?

The specific activity of an enzyme is expressed as the number of units per milligram of protein. The rate of a biochemical reaction at a given temperature and pH depends on the enzyme concentration and the substrate concentration.

What is the purpose of enzymes in plants?

Enzymes. Enzyme activity is usually the objective of growing bugs in a plant. Enzymes are biological catalysts. Just like other catalysts, they reduce activation energy, allowing reactions to proceed in a desired direction.

Where is the expression of a symbiotic enzyme located?

Expression of this enzyme is specific to the intestinal mucosa. The enzyme is mainly localized to the microvillar membrane in small intestine, in endosome-like structures, and in the Golgi complexes of human enterocytes. In addition, the decrease of the enzyme activity has been shown to be associated with colon cancer.

Why is there no additional being released when the enzyme activity slows down?

As the enzyme activity slows down (when the slope reaches zero), there is no additional being released because the cells start using up the for their own metabolism. As the enzyme activity slows down (when the slope reaches zero), there is no additional being released because the cells have used up the substrate.

When does the rate of product transport reach its maximum?

The rate of product transport reaches its maximum when the slope reaches zero, and that is why the most is released at the end of the time course. The enzyme activity level reaches its maximum when the slope reaches zero, and from there the activity remains steady because there is no more substrate.