o Selectable marker on our plasmid encodes a gene that expresses antibiotic resistance We are trying to make the cells resistant to ampicillin o Ampicillin binds to enzymes in the bacteria that are involved in synthesis of the cell wall and inhibits their function no bacterial cell wall and therefore they can’t divide Beta-lactamase: the gene that encodes an enzyme that catalyzes the …
What is the selectable marker in our plasmid? (1 point) kanamycin. kanamycin. 4. ... Lab Quiz 3 Questions Bio2296 Spring 2020.doc. 6. Quiz #3 Pool. Temple University. BIOL 2296. Genetics; Mitosis; ... Course Hero is not sponsored or endorsed by any college or university. ...
Mar 15, 2018 · What is the selectable marker in our plasmid? (1 point) ampicillin resistance 4) Give two similarities between plasmids & bacterial …
Know what a plasmid is and why it is important for a plasmid to have a selectable marker (know what a selectable marker is) - Plasmids are DNA molecules present within a cell, physically separated from a chromosomal DNA pGAL plasmid- replicates autonomously, does not integrate into the bacterial chromosome, contains the complete gene for B-galactosidase.
The genetic information contained in a haploid set of chromosomes is the genome of the species. During meiosis, the diploid number is converted into the haploid number. Haploid gametes contain just one member of each homologous pair of chromosomes, but following fusion of gametes, the diploid number is reestablished.
Bacterial chromosomes sometimes consist of single-stranded DNA. H1 and HU are two types of DNA-binding proteins found in bacterial chromosomes. (bacterial chromosomes are much more simple and H1 and H4 are found in eukaryotic chromosomes) T/F and WHY: Mitochondrial genes closely resemble eukaryotic nuclear genes.
Only one allele needed to exert phenotype, because with just one Oc allele, you will get 1 copy of Z no matter what, and 1 copy is enough for a normal phenotype. We have seen that the lac genes are expressed upon the condition that lactose is present in the media.
True. 50% of the human genome consists of transposable elements, but only 2% of the human genome comprises gene coding sequences. Even though most are inactive, the potential mobility and mutagenic effects of transposable elements have far reaching implications. Major differences between meiosis and mitosis.
hypermorphic because there is more expression and more gene product. 20 units of a gene's product, the protein fibrinogen, are needed for normal expression (i.e. enough for normal blood clotting). Each wildtype allele (R1) produces 25 units and a mutant allele (R2) produces 0 units.
A cis-acting element are adjacent parts on the same DNA molecule. Cis element is a DNA sequence that regulates expression of a gene located on the same chromosome and a trans-acting element is a gene product (usually protein or RNA) that acts to regulate the expression of the target gene.
D) In a DNA macromolecule, the two strands are complementary and antiparallel. (In a DNA macromolecule, the two strands are complementary and antiparallel. There is an axis of helical symmetry and the double helix twists around this axis. The tight packing of DNA bases causes this twist and causes base-stacking.