There are three components of a TLC system: the stationary phase, the solute, and the development solvent. The stationary phase is the surface on which the compounds will be separated, which is usually composed of extremely polar silica gel. The compounds or mixtures being analyzed are the solutes.
This layer of adsorbent is known as the stationary phase. After the sample has been applied on the plate, a solvent or solvent mixture (known as the mobile phase) is drawn up the plate via capillary action.
A TLC plate is a sheet of glass, metal, or plastic which is coated with a thin layer of a solid adsorbent (usually silica or alumina). A small amount of the mixture to be analyzed is spotted near the bottom of this plate.
Thin layer chromatography can be used to identify a compound because for certain reaction mixtures, TLC can separate all compounds in a mixture based on the distance the compound travels on the TLC film. With the proper solvent system, TLC can be used to uniquely identify a compound.
Thin Layer Chromatography can be defined as a method of separation or identification of a mixture of components into individual components by using finely divided adsorbent solid / (liquid) spread over a plate and liquid as a mobile phase.
Types of ChromatographyAdsorption Chromatography.Thin Layer Chromatography.Column Chromatography.Partition chromatography.
Thin layer chromatography (TLC) is a widely used separation technique for quantitative and qualitative analysis. It uses a thin layer of a stationary phase coated on a glass, plastic, or aluminum plate. A liquid solvent called the mobile phase carries the sample and separates it as it moves across the plate.
The principle of TLC is the distribution of a compound between a solid fixed phase (the thin layer) applied to a glass or plastic plate and a liquid mobile phase (eluting solvent) that is moving over the solid phase.
Thin Layer Chromatography (TLC) is a solid-liquid technique in which the two phases are a solid (stationary phase) and a liquid (moving phase). Solids most commonly used in chromatography are silica gel (SiO2 x H2O) and alumina (Al2O3 x H2O).
Thin-layer chromatography (TLC) is a very commonly used technique in synthetic chemistry for identifying compounds, determining their purity and following the progress of a reaction. It also permits the optimization of the solvent system for a given separation problem.
In TLC and column chromatography, the mobile phase is an organic liquid. In Gas Chromatography (GC) the mobile phase is a gas. a material used in chromatography which does not move. The mobile phase passes through the stationary phase.
TLC can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound. By observing the appearance of a product or the disappearance of a reactant, it can also be used to monitor the progress of a reaction.