how to do science course ware drosophila

How is culture media prepared for Drosophila?

3.2 Preparing Drosophila mediaThe commonly used media for various drosophilid stock maintenance includes Agar, yeast, Maize flour, Brown sugar, Nepagin, and Propionic acid.To prepare fly media agar is added to the hot water. ... Maize powder, brown sugar, and dried yeast are used as food.

How do you Aesthetize fruit flies?

0:051:33How To Anesthetize Drosophila - YouTubeYouTubeStart of suggested clipEnd of suggested clipQuickly remove the foam plug and insert the wand into the vial just below the plug exercise cautionMoreQuickly remove the foam plug and insert the wand into the vial just below the plug exercise caution not to touch the foam of the one containing Lola fly to the foam plug.

How do I get Virgin Drosophila?

Remove all flies 8-10 hours before collecting (generally this is done first thing in the morning). Visually inspect surface of food to ensure complete removal of flies. After 8-10 hours (usually before you leave work) collect all females that are present. All will be virgins.

How do you make flightless fruit fly culture?

0:433:43Culturing Wingless Fruit Flies (Drosophila melanogaster) as Live ...YouTubeStart of suggested clipEnd of suggested clipSet up whether you use commercial or do-it-yourself medium the mixture should be moist enough soMoreSet up whether you use commercial or do-it-yourself medium the mixture should be moist enough so that the surface of the medium quickly becomes smooth.

How do you culture a Drosophila lab?

1:222:49Culturing Drosophila Melanogaster - YouTubeYouTubeStart of suggested clipEnd of suggested clipSprinkle 5 to 6 grains of dry active yeast on top add flies to the bowel 1 to 2 minutes afterMoreSprinkle 5 to 6 grains of dry active yeast on top add flies to the bowel 1 to 2 minutes after hydrating. The media. If your goal is to simply maintain. Back up stop cultures.

How do you transfer Drosophila?

0:051:17Trapping & Transferring Fruit Flies - YouTubeYouTubeStart of suggested clipEnd of suggested clipQuickly remove the foam stopper. And invert the tube onto the funnel tap the vials on the table. SoMoreQuickly remove the foam stopper. And invert the tube onto the funnel tap the vials on the table. So that all the flies fall into their new tube.

How do you maintain Drosophila stocks?

Keeping Stocks Most stocks can be kept by dump-transfer of flies to fresh vials. However, it is important to avoid too overcrowded cultures, and only 20 or so flies should be transferred. It is good practice to inspect the flies on transfer, to ensure that both sexes are present and that their phenotype is as expected.

How long is a Drosophila life cycle?

about 10 daysThe life cycle of drosophila, from egg fertilization to adult life, takes about 10 days at 25°C. Drosophila is a model organism particularly used in developmental biology because it is a holometabolous insect, with major morphological differences occurring between larvae and adult animal (metamorphosis) [1].

How do you know if a fruit fly is virgin?

0:071:08Fly Basics 2: Virgins - YouTubeYouTubeStart of suggested clipEnd of suggested clipSo young female flies actually young male and female flies both are very pale. And whitish. AndMoreSo young female flies actually young male and female flies both are very pale. And whitish. And large they're bloated so in the lower left is a large female virgin fly when they're young and they look

How do you start a fruit fly culture?

1:553:35How to Culture Fruit Flies - YouTubeYouTubeStart of suggested clipEnd of suggested clipWhen you're first starting the culture fruit flies it takes a little bit of practice to transferMoreWhen you're first starting the culture fruit flies it takes a little bit of practice to transfer flies without getting them everywhere. So you can take the culture throw it in your fridge.

How do you make a fruit fly culture at home?

1:5911:09Homemade Fruit Fly media!! - YouTubeYouTubeStart of suggested clipEnd of suggested clipYou can use apple cider vinegar or you can use white vinegar either one works and then you'reMoreYou can use apple cider vinegar or you can use white vinegar either one works and then you're essentially just going to mix it after you have the vinegar.

How do you make fruit fly culture?

Put banana and sugar in blender and mix until the banana is liquified. Mix in oatmeal until it becomes firm, but still moist. Put mixture in wide mouth quart canning jars. Add a few granules of bakers yeast to the surface and add about 30 fruit flies.

What do scientists learn about fruit fly development?

However, scientists and geneticists have learned a lot about human development and genetics from studying the development of this model organism. In fact, many of the genes involved in fruit fly development are conserved through human development. Development of the common fruit fly, or Drosophila melanogaster, ...

How do fruit fly larvae develop?

Development of the common fruit fly, or Drosophila melanogaster, begins with an egg that becomes fertilized. This fertilized egg, or embryo, hatches into a larva. This larva feeds on the rotting fruit in your trashcan. And then, this larva develops into a pupa. This pupa eventually becomes what you know as the adult fly through metamorphosis.

What are the three sets of genes that are involved in proper pattern formation of the anterior to posterior axis?

The first set of genes is called the maternal-effect genes . The second set of genes is called the segmentation genes , and the third set of genes is known as the homeotic genes.

What is the anterior part of a fly embryo?

If we look at a fly embryo, the anterior is the part of the embryo that will develop into the head, while the posterior is the tail end of the embryo.

What are maternal effects in fly development?

First, maternal-effect genes encode for proteins that establish a concentration gradient across an embryo. In fact, products of these genes are in eggs even before fertilization occurs.

What is pattern formation in flies?

Pattern formation is the development of a body according to a specific and planned spatial arrangement. Pattern formation in fruit flies has been well studied by geneticists ...

Do fruit flies grow in garbage cans?

Although you have probably noticed a swarm of fruit flies around your garbage can when you have waited too long to take out the trash, you've probably not noticed their complex body structure or appreciated how an adult fly can be such an incredible example of animal development. Nobody blames you for that; flies are not exactly cute and cuddly. However, in this lesson, we'll start to learn how tiny fly larvae develop into adult fruit flies.

Insanely comprehensive list of phenomena for lesson planning

You're welcome. This is a ridiculously well-put-together list of phenomena, sorted by NGSS standards and grade level. Please don't spam them with edit access requests, just enjoy.

Black in STEM series

Each week I'm interviewing a Black person in STEM this week we talked to Mekalle Chapple I always make a quiz to go with the assignment so here that is too. Please reach out to me, if you know anyone that would want to talk to my students!

Any physics teachers want to share what has worked well while remote teaching?

My students are struggling and I'm not really sure how to as effective as I can be teaching remotely.

Improving scientific literacy

Hello teachers of reddit! It's been 3 years since i started teaching science. What I realize is that students aren't willing to explain their reasoning while answering questions. I think it is also because they don't feel comfortable about their literacy skills. How can I facilitate them to be better in their explanation? What would you suggest me?

Free access to all my lessons

So far my complete units are: States of Matter and Waves. I also have climate change, energy transfer, ecosystem, human impact and molecule lessons. I add more lessons weekly.

Help finding virtual labs

Hi all! I was wondering if anyone has any virtual lab resources for the earth sciences (geology, astronomy, meteorology) at the middle school level? I've seen a lot of cool virtual labs online, but they're mostly for biology and chemistry.