how to do a dihybrid cross simulation using science course ware drosophila

by Corbin Kunde 6 min read

What is a dihybrid cross in Drosophila?

Dihybrid cross in Drosophila Another instance of a cross that is a dihybrid can be seen in cases of homozygous long winged black-bodied Drosophila and grey-bodied, vestigial-winged fly. It is believed that the F1 generation hybrids are grey-bodied long-winged Drosophila fly.

How to do a dihybrid cross?

The very first step that you should complete when doing a dihybrid cross is to figure out the possible gametes of the parents. We must figure out all of the ways possible for the alleles to sort themselves based on Mendel’s second law of independent assortment. We will fill this part of the table out first.

What is the Drosophila simulation?

Students follow instructions within the simulation while they answer the lab questions on this page. Drosophila Simulation - Patterns of Heredity Objective: Students will learn and apply the principles of Mendelian inheritance by experimentation with the fruit fly Drosophila melanogaster.

What is the phenotypic ratio for a dihybrid cross?

Now we can create a ratio. The phenotypic ratio for this dihybrid cross will be 8 (black fur, short fur): 8 (black fur, long fur): 0 (brown fur, short fur): 0 (brown fur, long fur).

Definition of Dihybrid Cross

A dihybrid crossing is a gene-cross between people who have homozygous or heterozygous genotypes that have two distinct characteristics or traits. Dihybrid crosses differ from the monohybrid crossing that only involves one genetic character or characteristic.

Examples of Dihybrid Cross

A dihybrid is the cross between a pea plant homozygous that has round, yellow seeds as well as dried green seeds. Round yellow seeds have been represented by the RRYY alleles, while the green seeds that are wrinkled are represented by rryy. The gametes resulting from the two alleles are RY and the ry.

What is a dihybrid cross?

A monohybrid cross is when mating occurs between two individuals with different alleles at a single locus of interest. When we consider these problems, plants will often be the focus because their mating can be most easily controlled by scientists.

What is the first step in a dihybrid cross?

The very first step that you should complete when doing a dihybrid cross is to figure out the possible gametes of the parents. We must figure out all of the ways possible for the alleles to sort themselves based on Mendel’s second law of independent assortment. We will fill this part of the table out first.

What is Mendel's second law?

Mendel’s second law states that alleles of one gene sort independently of alleles of another gene. Basically, when performing a dihybrid cross, you can think of it as two separate monohybrid crosses.

What does it mean when a flower is homozygous?

We will produce 100% homozygous dominant plants. Homozygous means that the individual has two of the same alleles. Each of the offspring will be tall stemmed.

Is a short stem a dominant allele?

A tall stem is a dominant allele, and a short stem is a recessive allele (for the purpose of this example). Let’s review several different mating situations and discuss their outcomes. In this case, we are crossing a tall-stemmed flower and a short-stemmed flower.

Do monohybrids occur in science?

Many times during cross-breeding, scientists will have to deal with organisms that have two different alleles at two different loci. For example, a dihybrid cross would be between a tall (TT) and blue plant (BB) with a short (tt) and red plant (bb).

What is a drosophila?

Drosophila is an interactive simulation activity that enhances the traditional fruit fly laboratory experience. The experience takes place in a virtual environment where students have an unlimited ability to design experiments and analyze patterns of genetic inheritance to discover the principles of genetics.

Where is the entry page for Drosophila?

The entry page for Drosophila is found directly at: http://ScienceCourseware.org/vcise/drosophila/

What is order of flies?

In general, the student: (1) selects a male and female fly with specific traits, (2) mates these two parent flies, (3) observes and records the characteristics that are passed onto their offspring, and (4) analyzes the results and provides a hypothesis based on the outcome of the experiments.

How to calculate chi squared statistic?

The formula for calculating the chi-square (χ2) test statistic is: In this formula, you take observed number for each phenotype, Oi, subtract the expected number, Ei, square the difference, and divide the squared difference by the expected number. You sum the chi- squared terms for all of the phenotypes to obtain your test statistic.

How to access data from multiple crosses?

Data sets from multiple crosses are numbered and can be accessed from the pull down menu on top of the table. The gender, phenotype, number of flies, and the proportion of the total population are listed in a tabular format.

How are flies sorted?

The flies are automatically sorted based on their appearance, that is, their phenotype.

What are the skills needed to conduct scientific inquiry?

Abilities necessary to do scientific inquiry: • Identify questions and concepts that guide scientific investigations Design and conduct scientific investigations Use technology and mathematics to improve investigations and communications • Formula te and revise scientific explanations and models using logic and evidence Recognize and analyze alternative explanations and models Communicate and defend a scientific argument

What is hybridization in biology?

A hybridization is a breeding experiment between two organisms which are identical hybrids for 2 traits. In other words, a dihybrid cross is a cross between two organisms, with both being heterozygous for two different traits. The individuals in this type of trait are homozygous for a specific trait. These traits are determined by DNA segments ...

How are traits determined in hybridization?

These traits are determined by DNA segments called genes. In a hybridization, the parents carry different pairs of alleles for every trait. One parent carries homozygous dominant allele, while the other one carries homozygous recessive allele.

How many genes did Mendel study?

Later also, through the same process of dihybrid cross, Mendel studies the inheritance of two genes in plants.

What traits did Mendel take for crossing?

Mendel took a pair of contradicting traits together for crossing, for example colour and the shape of seeds at a time. He chose the green wrinkled seed and round-yellow seed and crossed them. He obtained only round-yellow seeds in the F1 generation.

What are the different types of seeds in the F2 generation?

This resulted in four different combinations of seeds in the F2 generation. They were wrinkled-yellow, round-yellow, wrinkled-green seeds and round-green within the phenotypic ratio of 9:3:3:1.

What is the phenotypic ratio of yellow and green?

During monohybrid cross of these traits, he observed the same pattern of dominance and inheritance. The phenotypic ratio 3:1 of yellow and green colour and of round and wrinkled seed shape during hybridization was retained in hybridization as well.

Monohybrid Cross

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In order to understand a dihybrid cross, we must first review a monohybrid cross. A monohybrid cross is when mating occurs between two individuals with different alleles at a single locus of interest. When we consider these problems, plants will often be the focus because their mating can be most easily controlled by scientis…
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Phenotypic and Genotypic Ratios

  • If the AP® Biology exam were to ask you about monohybrid crosses, the question will likely focus on the ratios that the cross produces. It will be necessary for you to first be able to fill out the Punnett square. After you have filled the Punnett square with the offspring genotypes, you can easily figure out the offspring ratios. Let’s look at the Punnett squares that we completed above…
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Theory of Dihybrid Cross

  • Monohybrid crosses do occur in science; however, organisms are much more complicated than one single allele. Many times during cross-breeding, scientists will have to deal with organisms that have two different alleles at two different loci. For example, a dihybrid cross would be between a tall (TT) and blue plant (BB) with a short (tt) and red plant (bb). It is important also to …
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Dihybrid Cross Problem

  • We are going to work through a dihybrid cross problem step-by-step. These problems can be complicated to learn, but when we have walked through one problem, you should be able to use the same method for all of the dihybrid problems. In guinea pigs, the allele for black fur (B) is dominant over that for brown fur (b). Similarly, the allele for short fur (S) is dominant over that fo…
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AP® Biology Exam Problem

  • Because these problems are a major sticking point for many students, and practice only makes these problems easier, we are going to do one more problem in this Dihybrid Cross: AP® Biology Crash Course Review. I won’t walk through all of the steps, but I will give you the problem and solution to see if you understand the dihybrid cross problems. Problem: Consider pea plants, in …
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Summary

  • In this article Dihybrid Cross: AP® Biology Crash Course Review, we began by reviewing the monohybrid cross and the possibilities that could result from crossing parents with different alleles. We worked through several monohybrid crosses and learned that we can find the genotypic and phenotypic ratios by using Punnett squares and creating fractions of every genot…
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