During a simple stain, what will happen in the heat fixatio omitted? This question was created from intro to micro module 4 learnsmart lab quiz.docx
Question 9 1 out of 1 points During a simple stain what will happen in the heat. Question 9 1 out of 1 points during a simple stain. School Rasmussen College; Course Title MICROBIOLO MCB2289; Type. Test Prep. Uploaded By DGirlMickey. Pages 7
B.) Heat fixation is performed, fixing the cells to the slide true to size. C.) The stains used dehydrate the cells, allowing for accurate measurement. D.) The cells are stained with a high contrast dye, allowing the borders to appear clearly. A.) …
What will happen if the heat fixation step is omitted? The cells may wash off of the slide.
What could happen if heat fixation was performed prior to doing a capsular stain? The heat might alter the morphology of the bacteria. A capsule stain was performed, using crystal violet as a primary stain, followed by a water rinse, and then nigrosin as a counterstain.
What is the purpose of heat fixation? Heat fixation ensures the elimination of contaminating organisms from the smear preparation. Heat fixation adheres the cells to the slide and coagulates the bacterial proteins, effectively killing the bacteria.
Heat-fixing the slide fixes the bacteria to the slide surface. If this step is not done, the bacteria in the smear would be washed off of the slide during the staining and decolorization steps.
The purpose of a simple stain is to allow visualization of bacteria by examination of the shape and arrangement. List 3 other dyes besides Methylene blue that can be used for simple staining.
The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.
The purpose of simple staining is to elucidate the morphology and arrangement of bacterial cells. The most commonly used basic stains are methylene blue, crystal violet, and carbol fuchsin.Jun 5, 2021
Fixation is often achieved either by heating (heat fixing) or chemically treating the specimen. In addition to attaching the specimen to the slide, fixation also kills microorganisms in the specimen, stopping their movement and metabolism while preserving the integrity of their cellular components for observation.
Simple staining allows one to observe the morphology and arrangement of the bacterial cells. Morphology refers to “form” or shape. We can use terms like spiral, bacilli (rod shaped), and cocci (spherical) to describe cell shapes.
Essentially, you will be using the clean slide to push the mixture across the surface of the slide. When you have finished spreading the slide, place the "clean" slide in a jar of disinfectant. Air dry the slide. DO NOT HEAT FIX.
If you heat the slide before it is completely dry, then you end up “boiling apart” the cells. The vapor pressure inside the cells will burst them… Heat fix the dried slide by quickly pulling it through a Bunsen burner (2x), but in a way that the cells do not touch the flame.
Heat fixing kills the bacteria in the smear, firmly adheres the smear to the slide, and allows the sample to more readily take up stains.