course hero a dna sequencing reaction contains which of the following

Why can't a dideoxynucleotide link to the next nucleotide?

Due to the structure of the dideoxynucleotide, it cannot link to the next nucleotide.

What happens at the end of a reaction?

At the end of the reaction, a series of fragments are produced in which the 5' ends are identical , and the 3' ends terminate at every A, G, C, or T residue.

How are the contents of all 4 tubes pooled and fractionated in a single lane?

Contents of all 4 tubes are pooled and fractionated in a single lane by electrophoresis.

What happens to DNA in gel electrophoresis?

During gel electrophoresis, DNA fragments can be loaded into one end of an agarose slab to separate the fragments on the basis of charge. As voltage is applied across the agarose slab, the DNA molecules, which have a negative charge, will migrate toward the positive electrode.

How many kilobase pairs are in a DNA fragment?

DNA fragments with an average size of 70 kilobase pairs (kb) after complete digestion of the DNA? The recognition sequence for each enzyme is indicated in the right-hand column.

What is the difference between cutting DNA with EcoRI and HaeIII?

Note that cutting DNA with EcoRI produces a staggered end, whereas cutting DNA with HaeIII produces a blunt end. Why must polymerase be added in this reaction?

How is DNA library constructed?

A DNA library has been constructed by purifying chromosomal DNA from mice, cutting the DNA with the restriction enzyme NotI, and inserting the fragments into the NotI site of a plasmid vector. What information cannot be retrieved from this library?

Why is DNA found furthest away from the area of the slab?

Because smaller DNA fragments will migrate more quickly, they will be found furthest away from the area of the slab where the DNA fragments were loaded. One method to visualize the DNA on the agarose slab involves staining the DNA with a dye that will fluoresce under ultraviolet light.

What is DNA ligase?

DNA ligase is an enzyme used when making recombinant DNA molecules in the lab. In what normal cellular process is DNA ligase involved?

Which nucleas cut DNA?

You have a linear piece of DNA that can be cut by the restriction nucleases HindIII and EcoRI, as diagrammed in Figure Q10-9.

What are the components of DNA sequencing reactions?

A DNA sequencing reaction includes four main ingredients, "Template" DNA copied by the E. coli; free bases, the building blocks of DNA that come in 4 types; short pieces of DNA called "primers"; and DNA polymerase, the enzyme that copies DNA.

What happens when a dye-bearing base attaches to the growing strand?

When a dye-bearing base attaches to the growing strand, it stops the new DNA strand from growing any further. A different colored dye is attached to each of the four kinds of bases.

What is the tail end of DNA?

The chemical reaction that makes DNA in a test tube is similar to what happens in a living cell: both rely on DNA polymerase and, in both cases, DNA strands have a head end, which is called the 5' end, and a tail end, which is called the 3' end. A DNA strand can grow only from its 3' end.

Can DNA grow from its 3' end?

A DNA strand can grow only from its 3' end. Making DNA in cells and sequencing DNA in test tubes both depend on complementary base pairing. The building blocks on opposite strands of DNA pair specifically - a C always pairs with a G, and an A always pairs with a T.